Consider extending transfer time or increasing voltage.Ĭonversely, small proteins migrate quickly and may be pushed through the membrane. Large proteins migrate more slowly out of the gel. Consider additional cooling or limiting transfer time.Ĭurrent increases when temperature increases. Limit transfer time to prevent small proteins from "blowing through" membrane.Ĭurrent increases when volume increases. Proteins move quickly through gels with low acrylamide percentages. Higher acrylamide percentages slow protein migration out of the gel. Consider additional cooling or limiting transfer time. See Our Western Blotting Transfer Membranes »Īddition of SDS or changes in ion concentration due to addition of acid or base, changes the resistance of the transfer system and therefore current and results in changes in current and voltage readings as well as transfer efficiency.Ĭurrent increases slightly as the number of gels increases. 0.2 μm pore size membranes are most suitable for transfer of low-molecular-weight ( ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |